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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2014 Apr 9;192(10):4610–4619. doi: 10.4049/jimmunol.1300692

Figure 5. Use of CD21/CD24 to compare emerging B cell pools during fetal/neonatal development in mice and humans.

Figure 5

Mononuclear cells were isolated from human cord blood (CB), human fetal spleen and pooled mouse fetal spleens. Cells were stained for flow cytometry to detect CD19, IgM, CD21, CD24, and in human samples for CD38, CD10, and CD5 as well. A-C, Cells falling in lymphocyte light scatter and that were CD19+IgM+ or IgM+ were gated into CD21/CD24 subsets. D-E, Subsets in human CB and human fetal spleens were evaluated for CD38, CD10 and CD5 expression. F-H, Graphed are the frequencies ±SD that each of the CD21/CD24 subsets contribute to the composition of the B cell pool. The mean ±SD for subsets in each tissue was derived from: n=10 human CB; n=6 human fetal spleens obtained at 18–23 weeks, (pregnancy date); and pooled mouse fetal spleen from C3H/HeN mice, day 18 of 20 day gestation, assayed in 3 independent experiments. Flow cytometry histogram and dot plots are representative data from indicated tissues.