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. 2014 May 3;10(5):530–542. doi: 10.7150/ijbs.8450

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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AGT mRNA localization in proximal tubule segment (S) 3 in OLETF and LETO rats. The merged images of AGT mRNA (A, D, G, and J) and the S3-specific marker ecto-ATPase (B, E, H, and K) are shown in C (LETO rats), F (OLETF rats), I (OLETF + olmesartan), and L (OLETF + HRH). The calculated AGT mRNA expression in S3 is shown in M. Each value was expressed as a fold-increase against the average of protein level of LETO rats, which was taken, which was taken as 1. ** P < 0.01 vs. LETO + vehicle. Magnification, x100 (scale bar, 200 μm). High magnification images of C, F, I, and L are also attached. AGT: angiotensinogen; LETO: Long-Evans Tokushima Otsuka; OLETF: Otsuka Long-Evans Tokushima Fatty; ecto-ATPase: ecto-adenosinetriphosphatase; HRH: hydralazine, reserpine, and hydrochlorothiazide.