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Dependence of (A) kcat/KM, (B) kcat, and (C) KM for isomerization of 5(10)-EST on the side chain pKa of substituted tyrosine. Tyr and F-Tyr containing variants are shown as closed circles; 3-Cl-Tyr is shown as open circles. Error bars correspond to standard deviations from 3 to 4 independent experiments using different enzyme concentrations. The slopes in parts A, B, and C are 0.03 ± 0.05, 0.07 ± 0.04, and 0.04 ± 0.04, respectively.
