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. Author manuscript; available in PMC: 2015 May 8.
Published in final edited form as: Cell Rep. 2014 Apr 24;7(3):907–917. doi: 10.1016/j.celrep.2014.03.065

Figure 3. Suppression of OXPHOS in Glycolytic Conditions Increases Energy Production.

Figure 3

(A) Electron transport chain schematic. Each subunit is colored according to the results of the RNAi screen in ATP/cell standard deviations from control siRNA.

(B) Results of the RNAi screen presented as percent change in ATP/cell for each subunit.

(C) Percent change in relative ATP/cell was determined compared to DMSO treatment. Measurements were obtained after 4 hours of treatment with ETC complex inhibitors (n=5 for each dose). Error bars represent SD values. *p < 0.05.

(D) Relative ATP/cell was measured in cells transfected with control or selected RC I subunit siRNAs and in cells treated for 500 nM rotenone for 4 hours (n=3 for each condition). Error bars represent SD values. **p < 0.01; ***p < 0.001.

(E) Glucose-to-lactate conversion in control and selected RC I subunit siRNA transfected HeLa cells. Cells were cultured with medium containing 1,6 13C-labeled glucose, and enrichment of 13C-lactate (m+3) in the extracellular medium was measured after 6 hours (n=3 for each condition). Error bars represent SD values. **p < 0.01; ***p < 0.001.