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. Author manuscript; available in PMC: 2015 May 8.
Published in final edited form as: Cell Rep. 2014 Apr 24;7(3):907–917. doi: 10.1016/j.celrep.2014.03.065

Figure 4. Mitochondrial Kinome and Phosphatome Impact on ATP Levels.

Figure 4

(A) Volcano plot depicting the results of a second RNAi screen targeting the mitochondrial kinome and phosphatome in glucose and pyruvate conditions.

(B) Relative ATP/cell was measured in cells transfected with control, AK4, or PPTC7 siRNAs. Error bars represent SD values (n=4 for each siRNA). **p < 0.01.

(C) Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured using a Seahorse Biosciences XFe96 Extracellular Flux Analyzer. Values were normalized to cell number. Cells were cultured in 10mM glucose, 1mM pyruvate, 1mM glutamine. Error bars represent SD values (n=10 for each siRNA).