Figure 3. SR inactivation impairs oxidative defenses.
A, B and C. SOD and catalase activity in biofilms as measured by native-protein activity gel staining (A) and biochemical assays (B and C). Error bars indicate SD. An image of intact gels from (A) are shown in Fig S11. P ≤0.001 (*), P ≤0.05 (**) versus the wild type.
D. Biofilms lacking HAQs show similar ofloxacin tolerance with or without an intact SR. Error bars indicate SD. P ≤0.001 (*) versus ΔpqsA.
E. Antibiotic tolerance in E.coli biofilms treated with ofloxacin (30 µg/mL) and tobramycin (50 µg/mL). Error bars indicate SD. P ≤0.005 (*) versus wild type.
