. Author manuscript; available in PMC: 2015 Feb 1.

Published in final edited form as: Prostaglandins Leukot Essent Fatty Acids. 2013 Dec 18;90(0):47–54. doi: 10.1016/j.plefa.2013.12.006

Table 1.

Oligonucleotide primers used in real time RTPCR assays
Gene	Forward primer	Reverse primer
FADS1	CCAGACTCCACTTCTCAA	GCCAGTTCACCAATCAGC
FADS2	Quanitect Primer Assay HS_FADS1_1_SG
ELOVl 2	Quanitect Primer Assay HS_ELOVL2_1_SG
ELOVL 5	Quanitect Primer Assay HS_ELOVL5_1_SG

Oligonucleotide primers used in DNA methylation assays
			PCR primers

Start CpG	End CpG	Length of amplicon (bp)	Forward primer	Reverse primer
-1661	-1665	168	GTATGGTGGTTTTGAGGATTGTT	AAAATACTCCCTAATTCTACCTTTCAACTA
-1337		290	TTGTTGTGAAATTTAGATTGGGTAGG	CCTAAAAAAAATAAACCTAACTACAT
-1278		290	TTGTTGTGAAATTTAGATTGGGTAGG	CCTAAAAAAAATAAACCTAACTACAT
-1156		90	TTGTTGTGAAATTTAGATTGGGTAGG	CCTAAAAAAAATAAACCTAACTACAT
-119	-1101	319	ATTTGAGGGTTTTATAATTTTTTTAGTGAT	ACCCTAATCTCAATAAACTCAATCT
-1071	-1056	218	ATTTGAGGGTTTTATAATTTTTTTAGTGAT	CCCTAATCTCAATAAACTCAATCTCTT
-1013	-975	323	ATTTGAGGGTTTTATAATTTTTTTAGTGAT	CCTTACCCTAATCTCAATAAACTCAATC
-914	-855	199	GGTAGTTTTTATTTGTTGGAGTTTGTAT	AAACCTCTACTCTACTTTCTTAATCT
-817	-775	167	ATTGAGTTTATTGAGATTAGGGTAAGG	ACTTTAAACCCTCTAATCAAACAATCTT
-718	-667	116	ATTGTTTGATTAGAGGGTTTAAAGTT	AAACTCCAATATCCCACATTAT
			Sequencing primers
			TGGTTTTGAGGATTGTTAA
			AGATTGGGTAGGGTT
			GGTTTTTTATTTTTAAGTGAGATG
			GGGTTTTATAATTTTTTTAGTGAT
			AAAATAAACCTAACTACATCC
			CCTCAAACCCCAACT
			ATTATACAAACTCCAACAAAT
			ATTATTGTTTAATGATGTGTTTG
			CCTCTAATCAAACAATCTTAAAA
			AGAGGGTTTAAAGTTTTTTAAT