Figure 4.
Survival with pyruvate fermentation in P. aeruginosa. Where shown, error bars indicate the standard error of three independent experiments. (A) Anaerobic survival for wild type PA14 (WT), PA14 ΔldhA (ΔldhA), PA14 ΔackA (ΔackA), and PA14 ΔackA-pta (ΔackA-pta) in the presence of 40 mM pyruvate (+pyruvate, closed points). WT PA14 without pyruvate is shown for comparison (−pyruvate, open points). 100% survival represents approximately 4 × 108 CFU/mL. Results are typical of least three independent experiments. (B) The [NADH]/[NAD+] ratio after 24 hours of pyruvate fermentation in PA14 WT, ∆ldhA, and ∆ackA. (C) ATP concentrations from the same experiments shown in panel B.
