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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Mol Microbiol. 2014 Mar 19;92(2):399–412. doi: 10.1111/mmi.12566

Figure 4.

Figure 4

Survival with pyruvate fermentation in P. aeruginosa. Where shown, error bars indicate the standard error of three independent experiments. (A) Anaerobic survival for wild type PA14 (WT), PA14 ΔldhAldhA), PA14 ΔackAackA), and PA14 ΔackA-ptaackA-pta) in the presence of 40 mM pyruvate (+pyruvate, closed points). WT PA14 without pyruvate is shown for comparison (−pyruvate, open points). 100% survival represents approximately 4 × 108 CFU/mL. Results are typical of least three independent experiments. (B) The [NADH]/[NAD+] ratio after 24 hours of pyruvate fermentation in PA14 WT, ∆ldhA, and ∆ackA. (C) ATP concentrations from the same experiments shown in panel B.