Figure 5.
Survival with the arginine deiminase pathway in P. aeruginosa. Where shown, error bars indicate the standard error of three independent experiments (A) Anaerobic survival for wild type PA14 (WT) and PA14 arcC::MAR2×T7 (arc::) in the presence of 40 mM arginine. WT PA14 without arginine is shown for comparison (−arginine, open points). 100% survival represents approximately 4 × 108 CFU/mL. Results are typical of at least three independent experiments. (B) The [NADH]/[NAD+] ratio after 24 hours of anaerobic incubation with arginine in PA14 WT and arcC::MAR2×T7. (C) ATP concentrations from the same experiments shown in panel B.
