Fig. 4.
Decreased oxygen tension increases intravasation of SW620 cells. (A) Fluorescent images of PVT spheroids cultured under 20% O2 and 5% O2 demonstrates that intravasation of SW620 is oxygen dependent. EC are labelled with CD31 antibodies (red). SW620 cells are transduced with EGFP (green). Scale bar represents 100 µm. (B) Confocal immunofluorescence analysis of single vessel branch reveals localization of tumor cells within lumens. Scale bar represents 20 µm. (C) Quantification of Migration Length and Migration Area shows the significantly enhanced intravasation of SW620 under conditions of low oxygen. Error bars represent standard deviation for each condition. **p < 0.001