Fig. 5.
SW620 intravasation is Slug-dependent. (A) SW620 cells incubated at 5% O2 showed more than a 5-fold increase in Slug expression compared to cells incubated at 20% O2. Values were normalized to GAPDH expression levels. (B) Slug knockdown efficiency in SW620 cells was validated by qPCR (top) and western blot (bottom) up to 72 hours post-transfection. (C) Quantification of Migration Length and Migration Area at day 7 confirms the attenuation of intravascular cell migration by siSlug compared to siControl. Error bars represent standard deviation for each condition. *p < 0.05 and **p < 0.001. NS: no statistically significant difference between conditions.