Table 1. Characteristics and primer sequences of the 6 genotyped VNTRs.
| Locus name | Repeated pattern | Chr.1 | Position2 | Primer sequence (5′→3′) | Ref |
| VC1 | AACAGA | 1 | 137106 | fw: CGGATACTCAAACGCAGGAT | [17], [18] |
| rv: 6FAM*-CTTTCGGTCGGTTTCTCTTG | |||||
| VC4 | TGCTGT | 2 | 187759 | fw: TGTTTGAGAGCTCGCCTCTT | [17], [18] |
| rv: PET*-TCATCAAGATGCACGACACA | |||||
| VC5 | GATAATCCA | 1 | 1915539 | fw: AGTGGGCACAGAGTGTCAAA | [17], [18] |
| rv: VIC*-AATTGGCCGCTAACTGAGTG | |||||
| VC9 | GACCCTA | 1 | 467111 | fw: CGTTAGCATCGAAACTGCTG | [17], [18] |
| rv: NED*-AGAAAACAATCGCCTGCTTG | |||||
| VCLAV6 | ACCAGA | 2 | 303939 | fw: NED*-GCCTCCTCAGAAGTTGAGAATC | Present study |
| rv: CCGATGAACTCTCTGAACTGG | |||||
| VCLAV8 | TTGTCGA | 1 | 532253 | fw: VIC*-CTCGCTTAAGTTGCCTTACCC | Present study |
| rv: GCGAACCAGACGTACTTTCAG |
1
Chr.: chromosome.
2
Based on the reference strain El Tor N16961 (GenBank accession numbers: AE003852.1 and AE003853.1).
PCR thermal cycling conditions for all assays: 95°C for 5 min; followed by 30 cycles of 95°C for 30 sec, 58°C for 30 sec and 72°C for 45 sec; 72°C for 5 min.