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. 2014 Jun 5;9(6):e97996. doi: 10.1371/journal.pone.0097996

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The assays were performed with the substrate mimic β-keto-butanoyl-SNAC. The product and substrate were separated with HPLC and monitored at UV 240 nm. (a) Assays with mutants at F123. (b) Assays with mutants at Y111. (c) Assays with mutants at F227. (d) Assays with mutants at Y235.