Figure 4. Ectopic Insb inhibits the expression of Notch targets.

(A) Expression of Insb in posterior (P) cells led to the reduced expression of the Notch target gene cut (Cut, green) and of the NRE-RFP reporter (RFP, red) in third instar wing imaginal discs (B–B”; compare with a wt control disc showing the expression of Cut and NRE-RFP in both anterior (A) and P cells along the dorsal-ventral boundary in A–A”). Scale bars are 20 µm. (A–B”).