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. 2014 Jun 5;9(6):e98213. doi: 10.1371/journal.pone.0098213

Figure 6. Insv and Insb may act independently of one another.

Figure 6

(A–B”) Insb-GFP (GFP, green) was specifically detected in the nucleus of SOPs (Sens, red), in 16 hrs APF insv mutant pupae. (C–C”) Ectopic expression of Insv in posterior (P) cells inhibited the expression of the Notch target gene cut (Cut, red) in wing discs without inducing the expression of Insb-GFP (GFP, green). The background red signal (star in C’) was generated by image projections due to a fold along A/P boundary in wing discs over-expressing Insv. (D–F) adult wings: wild-type (D), overexpression of Insb-GFP in wild-type (E) and insv mutant background (F) using sd-Gal4 driver. Loss of insv function had no effect on the Insb-GFP induced phenotype. Scale bars are 100 µm (A–A”), 5 µm (B–B”) and 20 µm (C–C”).