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. 2014 Jun 5;9(6):e98282. doi: 10.1371/journal.pone.0098282

Figure 3. Identification of mutations induced by ldlr TALEN.

Figure 3

(A) A schematic diagram showing primers for detecting mutations in TALEN target sites of ldlr gene. (B) The relative amplification efficiency of ldlr Pf primers to Po primers. ldlr TALEN mRNAs injection dramatically reduced the amplification efficiency of ldlr Pf primers. 5 embryos from the wild-type group or the ldlr-TALEN-injected group were pooled and analyzed by qPCR, and 3 pools of each group were analyzed in an independent experiment. Similar results were obtained in three independent experiments. (C) Identification of a mutation in a single allele with T-CIA. Upper panel was an agarose electrophoresis result of PCR products amplified with ldlr Po and Pf primers. Lower panel listed the sequences of mutant colonies identified in upper panel.