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. 2014 Jun 5;9(6):e98877. doi: 10.1371/journal.pone.0098877

Figure 4. NS3-specific CTL activity induced by DNA vaccination.

Figure 4

BALB/c mice (2 mice/group) were immunized with each of the DNA vaccines expressing NS3wt, various NS3 mutants or NS3/4A. Splenocytes obtained from the immunized mice were stimulated in vitro for 5 days with P815-NS3 cells and GST-NS3wt (5 µg/ml). Effectors and targets (P815-NS3) were cocultured for 4 h with the ratios of 50∶1, 25∶1, and 12.5∶1. Released LDH was measured and the percentage of specific killing was calculated. Specific CTL activity of splenocytes obtained from NS3-immunized mice and the mock-immunized control are shown with solid and dashed lines, respectively. Data represent mean ± SEM of the data from three independent experiments. *, p<0.01; †, p<0.05, compared with the mock-immunized control.