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BY2 cells that were expressed with SALI3-2-mGFP or mGFP were observed under confocal microscopy. Confocal fluorescence (green for mGFP, red for the membrane marked by fluorescent dyes FM4-64, and the merged images) and bright-field transmission are shown. As the control, the vacuoles are shown by neutral red staining. Scale bars = 10 µm.
