Figure 1. Effects of acute exercise on fibre-type specific glycogen depletion and phosphorylation of AMPKα.

A: Representative slides of immunofluorescent fibre-type analysis (blue fibres are type I, green are type IIA, red/green are type IIAX/IIX) with serial sections of glycogen (periodic acid-Schiff staining) and p-AMPKα Thr¹⁷² content before (Rest) and immediately after (Ex) an acute bout of END or LV-HIT. B–C: Fold change in glycogen content from Rest and Ex in type I and IIA fibres. D–E: Fold change in p-AMPKα content from Rest and Ex in type I and IIA fibres. Bars represent 100 µm. AU, arbitrary units; µm, micrometre. *Significant (p<0.05) main effect of exercise.