Table 2. Antibodies used in immunohistochemistry (IHC) and static cytometry studies.
| Antigen | Technique | Primary antibody | Antigen retrieval treatment* |
| CD68 | IHC | Monoclonal Mouse Anti-Human CD68 Clone PG-M1 (Dako) | α-Chymotrypsin (Sigma), 20 min, 37°C |
| CD86 | IHC | B7-2/CD86 (Epitomics) | Target Retrieval Solution pH 6 (Dako), 20 min, 95°C |
| Static cytometry | FITC Mouse Anti-Human CD86 (BD Pharmingen) | - | |
| CD206 | IHC | Anti-MRCI (Sigma) | Target Retrieval Solution pH 9 (Dako), 20 min, 95°C |
| Static cytometry | PE Mouse Anti-Human CD206 (BD Pharmingen) | - | |
| Gastrin | IHC | FLEX Polyclonal Rabbit Anti-Human Gastrin (Dako) | Target Retrieval Solution pH 9 (Dako), 20 min, 95°C |
| Wnt1 | IHC | Polyclonal Rabbit Anti-Wnt1 (Sigma) | Target Retrieval Solution pH 6 (Dako), 20 min, 95°C |
*Procedure to unmask the antigen.