Table 3. Primer sequences of specific PCR products for each gene analyzed.
Human Gene | Sense | Antisense | Length (bp) |
Wnt1 | 5′-CGCCCACCCGAGTACCTCCA-3′ | 5′-TTCATGCCGCCCCAGGCAAG-3′ | 110 |
Wnt3a | 5′-TACTCCTCTGCAGCCTGAAGCA-3′ | 5′-ATGGCGTGGACAAAGGCCGAC-3′ | 322 |
Wnt5a | 5′-CTGCCCCAACTCGGGAGTCCAGG-3′ | 5′-AGGAATCCGAGCGGAGCGACC-3′ | 147 |
Lgr5 | 5′-GGCTCGGTGTGCTCCTGTCCT-3′ | 5′-TGCCTCAGGGAATGCAGGCC-3′ | 484 |
β-actin | 5′-GGACTTCGAGCAAGAGATGG-3′ | 5′-AGCACTGTGTTGGCGTACAG-3′ | 67 |