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. 2014 Jun 5;9(6):e99001. doi: 10.1371/journal.pone.0099001

Figure 6. Cytochalasin D does not affect the quantal release in cells injected with the cSrc SH3 domain.

Figure 6

ACCs were injected with 5 µM GST or c-Src SH3-GST (SH3), and 20 min later incubated with 4 µM cytochalasin D (CytoD) or its vehicle DMSO for 10 min at 37°C. Then, exocytosis was induced with 20 µM ionomycin and monitored by amperometry. Data show average values ± S.E.M. of Imax, Q, t1/2, tP, foot duration, foot amplitude, foot frequency and number of events during the recording from cells injected with GST and treated with DMSO (n = 15) or CytoD (n = 16) or injected with SH3 treated with DMSO (n = 24) or CytoD (n = 14). All amperometric parameter values correspond to the median values of the events from individual cells, which were subsequently averaged per treatment group. *p<0.05 compared with cells injected with GST and treated with DMSO. Note that SH3 and CytoD have common effects in tP, foot duration and foot frequency, but show dissimilar effects on Imax and Q. In the latter parameters, the effects of c-Src-SH3 prevail over those of CytoD.