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. 2014 Jun 5;9(6):e98870. doi: 10.1371/journal.pone.0098870

Figure 3. Opsin expression in the rhopalium of Tripedalia cystophora.

Figure 3

In situ hybridization colorimetric staining places Tc-leo mRNA expression in the cell bodies of the retinal photoreceptors of the lens eyes (upper lens eye ULE and lower lens eye LLE) (A,B). The control with the sense probe (C) is devoid of colorimetric staining validating the positive results in A and B. Tc-neo mRNA is expressed in part of the neuropil (D,E), which is also known to have photosensitive properties [10]. Tc-neo sense control is seen in F. None of the opsins are expressed in the lesser eyes (pit eyes PE and slit eyes SE), suggesting that other opsins could be expressed in these eye types.