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. 2014 Jun 5;9(6):e99074. doi: 10.1371/journal.pone.0099074

Figure 5. FLZ domain mediates the interaction of FLZ1 and PFA-DSP3.

Figure 5

(A) BiFC, FLZ1 and PFA-DSP3 interact exclusively in nucleolus. Upper panel: whole cell, YFP alone, DAPI alone and merged. Lower panel: nucleus zoomed, YFP alone, DAPI alone and merged. (B) BiFC of PFA-DSP3 with deletion constructs of FLZ1: N-terminal (1–88 amino acids), FLZ (89–140 amino acids) and C-terminal (141–177 amino acids). (C) BiFC of PFA-DSP3 with AT5G49120: YFP alone, DAPI alone and merged. YFP were excited at 514 nm and emission was recorded at 530 nm. DAPI were excited at 351 nm and emission was recorded at 450 nm.