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. 2014 Jun 5;9(6):e99139. doi: 10.1371/journal.pone.0099139

Figure 8. MCMV gp48-mediated trafficking of class I MHC molecules is affected by depletion of AP-1μ or AP-3μ subunits.

Figure 8

Immunofluorescence analysis of class I MHC molecules in cells expressing gp48HA, − (a) and + (b) AP-1μ siRNA, and - (d) and + (e) AP-3μ siRNA, as indicated. Arrows in panels b and e point to the plasma membrane. Scale bar = 10 µm. c and f) Immunoblot analysis of AP-1μ or AP-3μ from lysates of U373-gp48HA cells −/+ treatment with AP-1μ (c) or AP-3μ (f) siRNA, as indicated. The Ponceau S stained nitrocellulose is shown beneath the immunoblots as a loading control.