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. 2014 Jun 5;9(6):e98853. doi: 10.1371/journal.pone.0098853

Figure 5. Upregulation of DYRK1A increases drug sensitivity of HL-60/ADM.

Figure 5

(A) HL-60/ADM cells infected with DYRK1A lentiviral particles(DYRK1A) or negative control(Control) were treated with gradient dilutions of doxorubicin for 72 hrs (0, 1, 2, 4 mg/L). Cell viability was measured by MTT assays. Results represent the mean ± S.E. from three independent experiments. *P<0.05. (B) HL-60/ADM cells infected with DYRK1A lentiviral particles(DYRK1A) or negative control(Control) were cultured in 0.5 mg/L doxorubicin for 48 hrs. Apoptosis was quantified by flow cytometry with dual staining of Annexin V-PE and 7-AAD. Dot plots of representative experiments showing apoptotic cell detection after 48 hrs of treatment with doxorubicin. Percentages of early (AV+7-AAD-) and late (AV+7-AAD+) apoptotic cells were calculated.