Figure 5. SOX2⁺ cells act as a glial progenitor in vivo.

A–B. After the spontaneous differentiation protocol of small spheres, the number of SOX2⁺ cells decreased, accompanied by the emergence of O4⁺ cell processes, which correspond to 58.35% ±21.11% of total cells. A small number of GFAP⁺ cells could be also detected (10.01% ±3.06%), all of them SOX2⁺. The scale bar represents 25 µm. C. SOX2⁺ population exhibits morphology compatible with oligodendroglial differentiation after the directed differentiation protocol, which was confirmed by the detection of myelin protein MBP. The scale bars represent 50 µm. D. No neuronal MAP2⁺ cells were observed in the spheres after the spontaneous differentiation of sphere-forming cells. Primary cell cultures derived from glioblastoma were used as a positive control. The scale bars represent 20 µm.