Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: FEBS J. 2014 Apr 22;281(11):2487–2502. doi: 10.1111/febs.12804

Figure 1. MPII and FRA3 constructs.

Figure 1

Top, wild-type MPII and FRA3. SP, PRO and CAT indicate the signal peptide, prodomain, and catalytic domain, respectively. The arrows indicate the cleavages at the prodomain excision sites. These cleavages release the prodomain and the mature protease. Bottom, the recombinant MPII and FRA3 constructs were tagged with the Hisx6 and FLAG tags. MPII-E352A and FRA3-E349A, the catalytically inactive mutants of MPII and FRA3, respectively. MPII-E352-T, the catalytically inert construct with the N-terminal Hisx6 tag followed by the thrombin cleavage sequence, was used in our crystallization studies.