Figure 6.
Residue segments predicted to promote or prevent amyloid formation mapped on the structure of lipid-free apoA-I monomer. Three N-terminal amyloid hot spots predicted in residues 14-22, 53-58, and 69-72 are mapped on the crystal structure of free Δ(185-243)apoA-I [10]. The fourth predicted “hot spot”, 227-232, is in the flexible C-terminal region (irregular line) that forms the primary lipid binding site in apoA-I driving its adsorption to phospholipid surface [18]. Residues 76-81 that are expected to form a β-breaking motif at pH 7 are in purple. Extended segment 44-55 is in red.