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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Leukemia. 2013 Dec 13;28(6):1259–1270. doi: 10.1038/leu.2013.373

Figure 5. Expression of lineage markers and genes in Gata1-/ Gata1s-transduced HSPCs.

Figure 5

(a) Heatmap of the normalized enrichment score (NES) obtained by GSEA of Gata1-, Gata1s- and empty vector control (ctr)-transduced fetal HSPCs grown 4 days (and 14 days) in myeloid differentiation medium compared to empty vector control (ctr)-transduced cells on day 0 (n=2). Gene sets specific (more than 2-fold, PANOVA<0.05) for human monocytes, granulocytes, megakaryocytes, erythroid cells and CD34+-HSPCs (Emmrich and Klusmann, unpublished data), and specific for eosinophils and mast cells (39) were used. The corresponding enrichment plots and statistics are presented in Supplementary Figures 8–11. (b) The expression of key erythroid, monocytic, eosinophil and basophil genes was validated by qRT-PCR in replicates from another independent experiment (mean±SD of replicates; *PANOVA<0.05, **PANOVA<0.01, ***PANOVA<0.001 compared to ctr). (c) ChIP-seq density plots of Gata1 (blue) and Gata1s (red) enrichment on promoter regions of eosinophil genes EPX and CLC.