Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
|
Plasmids |
|
|
| pUC18 |
Ampr, lacZ', pMB9 replicon |
[13] |
| pUC18::ESA_02131 |
pUC18 with ESA_02131 gene under control of native promoter |
This work |
| pUC18::ESA_01706 |
pUC18 with ESA_01706 gene under control of native promoter |
This work |
| pUC18::ESA_04214 |
pUC18 with ESA_04214 gene under control of native promoter |
This work |
| pUC18::ESA_pESA3p05450 |
pUC18 with ESA_pESA3p05450 gene under control of native promoter |
This work |
| pUC18::ESA_01226 |
pUC18 with ESA_01226 gene under control of native promoter |
This work |
| pUC18::ESA_00673 |
pUC18 with ESA_00673 gene under control of native promoter |
This work |
| pUC18::ESA_03328 |
pUC18 with ESA_03328 gene under control of native promoter |
This work |
|
Strains |
|
|
|
Cronobacter sakazakii BAA-894 |
C.sakazakii strain isolated from powdered formula associated with neonatal intensive care unit |
[12] |
|
Escherichia coli DH5α |
Intermediate cloning host.supE44 ΔlacU169(80lacZΔM15)R17 recA1 endA1 gyrA96 thi-1 relA1. |
Invitrogen. |
| MKH13 |
MC4100Δ(putPA)101Δ(proP)2Δ(proU) |
[15] |
| MKH13 pUC18::ESA_02131+ |
Host strain harbouring pUC18: ESA_02131 plasmid. Ampr. |
This work |
| MKH13 pUC18::ESA_01706+ |
Host strain harbouring pUC18: ESA_01706 plasmid. Ampr. |
This work |
| MKH13 pUC18::ESA_04214+ |
Host strain harbouring pUC18: ESA_04214 plasmid. Ampr. |
This work |
| MKH13 pUC18::ESA_pESA3p05450+ |
Host strain harbouring pUC18: ESA_pESA3p05450 plasmid. Ampr. |
This work |
| MKH13 pUC18::ESA_01226+ |
Host strain harbouring pUC18: ESA_01226 plasmid. Ampr. |
This work |
| MKH13 pUC18::ESA_00673+ |
Host strain harbouring pUC18: ESA_00673 plasmid. Ampr. |
This work |
| MKH13 pUC18::ESA_03328+ | Host strain harbouring pUC18: ESA_03328 plasmid. Ampr. | This work |
Ampr - This strain is resistant to ampicillin.