Table 2.

Primers

Primer name		Primer sequence (5' to 3')	Length	Characteristics
ESA_02131	F	CATCGGCCGACAGGCCAGTCAATGAATGATGC	32	Eag1cut site
	R	CATTCTAGAGAGTACAACGGAATGCGGGG	29	Xba1 cut site
ESA_01706	F	CATTCTAGAGTCGGGCGGCTCTTTATCTGG	30	Xba1 cut site
	R	CATGGATCCTTGACCAGATGACGCAGTCTTTC	32	BamH1 cut site
ESA_04214	F	CATGAATTCGTCTCTTTCTGTGCCAACTATCTGC	34	EcoR1 cut site
	R	CATTCTAGACTACCTGACGCGTACCCTGTATATC	34	Xba1 cut site
ESA_pESA3p05450	F	CATGAATTCATCATCTCTACACGCTGCCTTCTG	33	EcoR1 cut site
	R	CATTCTAGATCTCCACCTGCGCCTCTATC	29	Xba1 cut site
ESA_01226	F	CATGAATTCCAGTGCGCCGGAGCTTTTCG	29	EcoR1 cut site
	R	CATTCTAGAGGGCTGTCGGTTGACGAAATTAAAC	34	Xba1 cut site
ESA_00673	F	CATGAATTCTAAAAGCGAAATCCTCCCGTACTGGC	35	EcoR1 cut site
	R	CATGGATCCCCTGCAAAGCATCGCCGATTACC	32	BamH1 cut stie
ESA_03328	F	CATTCTAGATCGCTATCGCTGACCGTGAAATG	32	Xba1 cut site
	R	CATGGATCCTGCTGAACGAACAGTATGGCCG	31	BamH1 cut site
pUC18 MCS Check	F	CATTAG CTC ACT CAT TAG GCA CC	20	pUC18 insert check
	R	CATTGT AAA ACG ACG GCC AGT G	19	pUC18 insert check
RTESA_02131	F	GCTGGCGTGTATCGGTCT	18	Probe 31
	R	CTC GGC ATA TAA GTA AGC AGC AT	23	Probe 31
RTESA_01706	F	CGA CGG TCA TTC TGC TCA C	19	Probe 32
	R	AGC AGG CCA ATC TGA TGG TA	20	Probe 32
RTESA_04214	F	GCA TAA ACG CGC CCT GTA	18	Probe 18
	R	TTA GCG AAG AAG AAG CCG ATA	21	Probe 18
RTESA_01226	F	GGG TAT CAG GTG GCA AGC	18	Probe 70
	R	GTA AAT CGC GAC CGT ATG C	19	Probe 70
RTESA_00673	F	CCG GAC AGA TAA ACC GTC AC	20	Probe 11
	R	GAC GAG GCA CCG ACA ATC	18	Probe 11
RTESA_03328	F	CGT GCC GTT CGT AAT GGT	18	Probe 29
	R	AAC GAA ATG CCG GTA AAG C	19	Probe 29
RTESA_pESA3p05450	F	TGG TGG CGA TTT CCA ACT	18	Probe 71
	R	CCC GAA ACG GTC AGA AAG	18	Probe 71
RT16S	F	TGT AGC GGT GAA ATG CGT AG	20	Probe 65
	R	AGC GTC AGT CTT CGT CCA G	19	Probe 65