Skip to main content
. 2014 Apr 24;289(23):15904–15914. doi: 10.1074/jbc.M114.555631

FIGURE 2.

FIGURE 2.

Construction and verification of a neutral site platform for Synechococcus sp. PCC 7002. A, diagram showing the construction of a SynPCC7002_A2746 mutant and positions of oligonucleotide primers (see Table 1). B, agarose gel electrophoresis of amplicons produced by polymerase chain reaction, demonstrating complete segregation of alleles for SynPCC7002_A2746::aphII and SynPCC7002_A2746, using primer set A2746upF and A2746downR. The template DNAs were isolated from the SynPCC7002_A2746 mutant (lane 1) and wild type (lane 2). C, comparison of growth rates for Synechococcus sp. PCC 7002 WT and a neutral site mutant strain constructed in open reading frame SynPCC7002_A2746 (ΔA2746). The growth rates were indistinguishable within experimental error. The data are the average of three biological replicates.