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. 2014 Apr 22;289(23):15957–15967. doi: 10.1074/jbc.M114.562686

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Radio-TLC analysis of the Ta1305 product from MVA and ³²P-labeled ATP. A, nonlabeled MVA and [γ-³²P]ATP were reacted with purified Ta1305 or S. solfataricus MVK. The products were analyzed by normal-phase TLC developed with CHCl₃/pyridine/formate/H₂O (12:28:6:4) and followed by autoradiography. B, nonlabeled MVA and [α-³²P]ATP were reacted with purified Ta1305 or S. solfataricus MVK. The products were analyzed by normal-phase TLC developed with propanol-1, methanol, 25% ammonium water (45:15:40). ori., origin; s.f., solvent front.