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Conversion efficiency of intermediates of the MVA pathways in the reaction with T. acidophilum cell-free extract. Radioactive intermediates including [2-14C]MVA-3-P were used for the assay. Their conversion into IPP was confirmed by the use of a prenyl-elongation reaction with S. acidocaldarius GGPP synthase. Conversion efficiency was calculated by dividing the radioactivity extracted in an n-pentane layer by that of an added intermediate. ori., origin; s.f., solvent front.
