FIGURE 2.

CPR3 deletion does not affect the yeast permeability transition. A, the experimental conditions were as in Fig. 1 with 2 mm P_i; 0.8 μm CsA was added in trace b only, and 2 μm rotenone was added in trace d only. Where indicated, Ca²⁺ was added to wild-type (traces a, b, and d) or ΔCPR3 (trace c) mitochondria (traces are representative of three independent experiments). B, the experimental conditions were as in Fig. 1 with P_i as indicated (n = 4 ± S.E.). Closed symbols, wild-type mitochondria; open symbols, ΔCPR3 mitochondria. Two-way analysis of variance test was performed, *, p < 0.05.