TABLE 2.

Thermodynamic parameters for inhibitors of ecFabI^a

^a Curve fitting errors are reported for each value in the table.

^b [S]/K_S = 0.19.

^c Value is reported against E. coli MG1655 ΔacrAB, a strain with a knockout of the specific efflux pump AcrAB.

^d n.d. means not determined.

^e The MIC value against E. coli MG1655 with the intact AcrAB efflux pump is >425 μm.

^f This binds to the E-NAD⁺ binary complex. K_i and K_i^app values were determined via single point progress curve analysis, as described in Ref. 31. Rationally derived kinetic parameters for each step in the mechanistic model depicted in Fig. 2B are listed in supplemental Table S3.

^g MIC values against E. coli MG1655 with the intact AcrAB efflux pump were 10–30-fold higher.