FIGURE 6.

Endorepellin induces autophagy through a VEGFR2-dependent pathway and evokes transcription of BECN1 gene. A, representative immunoblotting following a 6-h treatment with endorepellin (200 nm) of HUVEC treated with scrambled siRNA (siScr), siRNA targeting the VEGFR2 (siVEGFR2), or SU5416 as indicated. The blotting is representative of three independent experiments with similar results. B, representative luciferase reporter assays of PAE-VEGFR2 ^BECN1-Luc cells treated with soluble endorepellin (200 nm) for the designated times, in the presence or absence of SU5416 (30 μm). Data are mean ± S.E., normalized to total cell protein. The values at 4 and 6 h are statistically significant (***, p < 0.001) as compared with time 0 and the SU5416-treated samples.