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. 2014 Apr 15;289(23):16114–16128. doi: 10.1074/jbc.M114.556530

FIGURE 7.

FIGURE 7.

p62 expression is affected by endorepellin promoting its relocalization to LC3-positive autophagosomes in HUVEC. A, representative immunoblotting showing a time course induction of p62 upon endorepellin treatment (200 nm), with GAPDH as control. B, quantification of p62 levels evoked by endorepellin (200 nm) treatment for various intervals as indicated. The values represent the mean ± S.E. of six independent experiments; *, p < 0.05; **, p < 0.01. C, immunofluorescence images of HUVEC treated with endorepellin (200 nm) for 6 h showing a relocalization of p62 (red) into LC3-positive autophagosomes. Nuclei are stained with DAPI (blue). Bar, 10 μm. D, immunofluorescence images of p62 (red) and LC3 (green) co-localization in HUVEC upon a 6-h treatment with vehicle (control), LG1/2 (150 nm), or LG3 (150 nm). Nuclei are stained with DAPI. Bar, 10 μm.