TABLE 1.
Biological titers of vectors used
Various vectors were produced according to the described methods. Biological titer was determined by serial dilution of vectors in HEK293T cells. The number of cells expressing β-galactosidase was determined 48 h post-transduction by manual counting. LFU, LacZ-forming units.
Vector | Biological titer (mean ± S.E.) |
---|---|
LFU/ml | |
RV-G.HIV-1 | 3.59 ± 0.97 × 107 |
RV-G.HIV1-DiO | 5.1 ± 2.11 × 107 |
RV-G.HIV1-DiD | 7.4 ± 1.87 × 107 |
RV-G.HIV1-IN-GFP | 1.1 ± 0.4 × 106 |
RV-G.HIV-1 (FlAsH) | 2.29 ± 0.91 × 107 |
RV-G.HIV1-Cys4-MA-C (FlAsH) | 1.72 ± 0.38 × 107 |
VSV-G.HIV-1 | 8.59 ± 3.4 × 107 |
RV-G.EIAV-Cys4-MA-C (FlAsH) | 9.47 ± 0.13 × 106 |