FIGURE 2.

Effect of GnRH on GDF9 mRNA synthesis rate and stability. A and B, LβT2 cells were serum-starved overnight, stimulated with 1 nm GnRH, and harvested at the indicated times. One-way ANOVA was used. C, LβT2 cells were pretreated for 10 min with either 2 μg/ml actinomycin D (ActD; 1.6 μm) or vehicle (control), stimulated with 5 nm GnRH or vehicle for 45 min. D, LβT2 cells were exposed to 2 μg/ml actinomycin D (1.6 μm) in either the presence or absence of 5 nm GnRH and harvested at the indicated time points. CGA and GDF9 mRNA (A and D, respectively) and their primary transcript levels (B), as well as c-jun mRNA levels (C) were determined by qPCR. The data shown are mean ± S.E. (error bars) of triplicate samples from one experiment and are representative of three independent experiments. Two-way ANOVA with Bonferroni post test corrections was used. ***, p < 0.001. A.U., arbitrary units.