FIGURE 12.

Monitoring superoxide radical anion in N27 cells after treatment with 6-hydroxydopamine. N27 cells were treated with 6-OH-DA (100 μm) for 6 h, followed by a 1-h treatment with NBT (0.3 mg/ml) (a) or 10 μm HE (b). a, UV-visible absorption spectra of the cell extracts after treatment with NBT. The inset shows the relative amount of the formazan formed, as determined based on the absorbance measured at 685 nm. b, profiles of HE oxidation determined in the cell lysates. Data shown in a and b are averages of three independent experiments. Error bars, S.D.