FIGURE 3.

Monitoring Nox2 activity of dHL60 cells. a, effect of SOD (0.1 mg/ml) and CAT (1 kilounit/ml) on the time-dependent oxidation of HPr⁺ induced by stimulation of dHL60 with PMA (1 μm). b, same as a, but CBA was used instead of HPr⁺. c, same as a, but using Amplex Red/HRP probe. d, HPLC traces of the media from incubation of dHL60 with PMA (1 μm) for 90 min in the presence of HE (20 μm). e–h, same as a–d, but the effect of DPI and VAS2870 inhibitors (10 μm each) was tested. i, OCR measurements; response to PMA of ndHL60 versus dHL60 cells. j, same as i, but the effect of DPI (10 μm) on basal and PMA-induced OCR in dHL60 cells was tested. k, same as j, but the effect of VAS2870 (10 μm) was tested. l, same as j, but the effect of rotenone (1 μm) + antimycin (10 μm) was tested. a.u., arbitrary units. Error bars, S.D.