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. 2014 Apr 24;289(23):16176–16189. doi: 10.1074/jbc.M114.548693

FIGURE 5.

FIGURE 5.

Simultaneous monitoring of superoxide radical anion and hydrogen peroxide in cells enriched with Nox2, Nox4, and Nox5, using a high-throughput HPLC method. a, the concentration of 2-OH-E+ and COH detected in the media of dHL60 cells stimulated with PMA (1 μm) in the presence of 20 μm HE and 100 μm CBA and in the presence and absence of SOD (0.1 mg/ml) or CAT (0.1 kilounit/ml). b, same as a, but HPLC traces of samples analyzed after 2 h incubation are shown. c and d, same as a and b, but HEK and HEK-Nox4 cells were used. e and f, same as a and b, but HEK-Nox5 cells were used. Ionomycin (1 μm) was used to stimulate production of O2⨪ and H2O2.