TABLE 1.

Fluorescence settings used in primary and orthogonal assays for O₂^⨪ and H₂O₂

Probe	Species detected	Product detected	Fluorescence detection settings
			Emission λmax	Excitation λmax
			nm
Primary assays
HPr+ + DNA	O2⨪	2-OH-Pr2+ bound to DNA	485	574
CBA	H2O2	COH	355	460
Orthogonal assays
HE	O2⨪	2-OH-E+ detected by HPLC	370	565
Amplex® Red + HRP	H2O2	Resorufin	535	595