TABLE 2.

Z′ values of the primary and orthogonal assays

Assay/Probe	Detection mode	Type of analysis	Z′
Primary assays
HPr+ + DNA	Plate reader	Kinetic	0.60 ± 0.17
	Plate reader	End point	0.55 ± 0.11
CBA	Plate reader	Kinetic	0.67 ± 0.15
	Plate reader	End point	0.68 ± 0.14
Orthogonal assays
HE	HPLC	End point	0.75 ± 0.07
Amplex Red + HRP	Plate reader	Kinetic	0.73 ± 0.11
	Plate reader	End point	0.74 ± 0.11