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. 2014 Apr 17;289(23):16200–16213. doi: 10.1074/jbc.M114.548115

FIGURE 3.

FIGURE 3.

AdipoR2 is necessary for the revascularization actions of adiponectin. A, wild-type and AdipoR2-KO mice were administered control (pLEV113-MCS) or adiponectin plasmid (pLEV113-mADIPO-hFc fusion construct, LakePharma) by hydrodynamic delivery (HD) 1 week before hind limb ischemia surgery. Adiponectin (APN) levels were measured by ELISA in serum samples harvested at the time of surgery. Data are the mean ± S.E. n = 5–9. *, p < 0.05 versus control treatment. Representative native Western blot of adiponectin isomers in serum harvested from mice at the time of surgery. HMW, high molecular weight adiponectin. B, representative Doppler images pre-surgery, immediately post-surgery, and 14 days post-surgery. C, laser Doppler perfusion analysis of control or adiponectin plasmid-treated wild-type mice. Data are the mean ± S.E. n = 6–9, *, p < 0.05. D, laser Doppler perfusion analysis of control or adiponectin plasmid-treated AdipoR2-KO mice. Data are the mean ± S.E., n = 5–7, p > 0.05.