Tissue mRNA expression analysis of candidate adiponectin receptors.
A, tissue distribution of AdipoR1, AdipoR2, and T-cadherin was assessed in liver, skeletal muscle (gastrocnemius (GC)), heart, epididymal white adipose tissue (WAT), brown adipose tissue (BAT), and aorta by measuring relative mRNA expression. Data are the mean ± S.E. n = 6. B, ischemic and non-ischemic gastrocnemius muscles were harvested at the following time points after hind limb ischemia: day 3 (n = 6), day 7 (n = 5), and day 14 (n = 3). Expression of AdipoR1, AdipoR2, and T-cadherin mRNA was measured at each time point. Data are the mean ± S.E. *, p < 0.05. C, relative mRNA levels of the candidate adiponectin receptors in tissue isolated from wild-type mice fed a normal diet (ND) or high fat/high sucrose (HFHS) diet for 12 weeks. Data are the mean ± S.E. n = 6. *, p < 0.05.