FIGURE 4.

A super-low dose of LPS promotes mitochondrial fission while simultaneously decreasing mitochondrial fusion. A, WT and IRAK-1-deficient BMDMs were treated with a super-low dose of LPS (50 pg/ml) for the indicated times. The levels of phosphorylated Drp1 (p-Drp1, Ser-637) were determined by Western blot analysis. The blots were probed with β-actin as loading controls. B, WT and IRAK1-deficient BMDMs were treated with a super-low dose of LPS (50 pg/ml) for the indicated times. The levels of Mfn1 were determined by Western blot analysis. The blots were probed with β-actin as loading controls. Band intensities of phospho-Drp1 (A) and Mfn1 (B) were quantified and normalized relative to the β-actin levels. All data are representative of three experiments and are represented as mean ± S.D. *, p < 0.05.