FIGURE 6.

A super-low dose of LPS promotes Mfn1 degradation. A, WT macrophages were treated with 50 pg/ml LPS in the absence or presence of the proteasome inhibitor MG132, followed by immunoblot analysis of Mfn1 protein levels. The blots were probed with β-actin as loading controls. DMSO, dimethyl sulfoxide. B, immunoprecipitation (IP) analysis was performed using cell lysates derived from WT and IRAK1-deficient macrophages treated with or without 50 pg/ml LPS with either an isotype control or Mfn1-specific antibodies, as indicated on the blots. The gels were immunoblotted (IB) with either ubiquitin-specific (Ub, top row) or Mfn1-specific (bottom row) antibodies. All data are representative of three experiments.